Epac1 Alleviates Senescence in Auditory Hair Cells via the Ferroptosis
Wen-Jun An, Chen-Yu Xu, Meng-Ya Shen, Xiao-Tao Guo, Chun-Chen Pan, Jing-Wu Sun, Xiao-Min Tang, Jia-Qiang Sun
Journal：World Journal of Otorhinolaryngology-Head & Neck Surgery
IF：1.4
DOI：10.1002/wjo2.70090
PMID：
Published：2026-02-09
research field：分子生物学听力学细胞生物学衰老研究
Abstract
Objectives The aim of this study was to investigate the changes in Epac1 and ferroptosis-related proteins in aged mice and the House Ear Institute-Organ of Corti 1 (HEI-OC1) cells, with the goal of elucidating their potential role in age-related hearing loss. Methods The expression of Epac1 in the cochlea of C57BL/6J mice was examined by using immunofluorescence staining and Western blot analysis. To further verify the role of Epac1 in age-related hearing loss, small interfering RNA (siRNA) was transfected into HEI-OC1 cells, resulting in the downregulation of Epac1. Results The results revealed a decrease in Epac1 expression in the cochlea of aged mice. After downregulating the level of Epac1 in vitro, a greater level of ferroptosis was observed, and the aging process was accelerated. Conclusions Epac1 plays a crucial role in the aging process of auditory hair cells via ferroptosis.
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