推荐应用
Recombinant full-length human CCR8 protein was expressed by the intact DNA sequence of the human CCR8 in mammalian cells. This recombinant CCR8 protein was fused with a 10xHis-tag at the C-terminus. It contains less than 1.0 EU/ug determined by the LAL method. The CCR8 protein is biologically active as it can react with the mouse anti-6xHis monoclonal antibody and anti-CCR8 recombinant antibody, respectively. Its VLP-like structure was observed and confirmed by TEM. This recombinant CCR8 protein is highly immunogenic because it carries viral protein. Past studies have shown that the CCR8 protein is mainly expressed on monocytes, Th2 cells, and Treg cells. Many basic studies have also proved CCR8 plays an essential role in the biological processes of lymph node migration of monocyte-derived dendritic cells and Th2 cell-mediated type 2 inflammation. Researchers have found that CCR8 is a good biomarker of Treg cells at tumor sites and a potential tumor immune target.
高纯度、高活性、低内毒素、高批间一致性
Western Blot
The Protein is detected by Mouse anti-6*His monoclonal antibody.
Activity
Measured by its binding ability in a functional ELISA. Immobilized human CCR8 at 5 μg/ml can bind Anti-CCR8 recombinant Antibody, the the EC50 is 11.13-17.29 ng/ml.
Western Blot
The Protein is detected by Anti-CCR8 recombinant Antibody.
The presence of VLP-like structures was confirmed by TEM
BLI assay
Loaded Anti-Human CCR8 Antibody on 96-Flat plate, can bind Human CCR8, with an affinity constant of ＜1 pM as determined in BLI assay (Gator Prime).
-25 ~ -15℃保存，收到货之后有效期1年。
