推荐应用
Coronaviruses are commonly comprised of four structural proteins: Spike protein (S), Envelope protein (E), Membrane protein (M) and Nucleocapsid protein (N) . The SARS-CoV-2 S protein is a glycoprotein that mediates membrane fusion and viral entry. The S protein is homotrimeric, with each ~180-kDa monomer consisting of two subunits, S1 and S2 . The RBD of SARS-CoV-2 binds a metallopeptidase, angiotensin-converting enzyme 2 (ACE-2). First detected in India in December 2020, the B.1.617.2, or Delta variant, is considered a Variant of Concern (VOC) as it contains several mutations in the RBD domain that potentially affect viral fitness and transmissibility: L452R and T478K.
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别名 |
S protein RBD; S glycoprotein RBD; Spike protein RBD |
|
Accession |
QHD43416.1 |
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表达区间及表达系统 |
Recombinant SARS-COV-2 Spike RBD (Delta B.1.617.2) Protein is expressed from Expi293 with His tag at the C-terminal. It contains Arg319-Phe541(L452R, T478K). |
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分子量 |
Approximately 26.26 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on Tris-Bis PAGE result. |
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纯度 |
> 95% as determined by SDS-PAGE and HPLC. |
|
活性 |
ELISA Data：Immobilized SARS-CoV-2 Spike RBD (Delta B.1.617.2) , His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 9.8ng/ml determined by ELISA. |
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内毒素 |
< 1.0 EU per 1μg of the protein by the LAL method. |
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制剂 |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). |
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复溶 |
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
