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The NF-kappa-B (NFKB) complex of proteins is inhibited by I-kappa-B (IKB) proteins, which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the IKB proteins by IKB kinases marks them for destruction via the ubiquitination pathway, thereby allowing activation and nuclear translocation of the NFKB complex. The protein encoded by this gene is similar to IKB kinases and can mediate NFKB activation in response to certain growth factors. The protein is also an important kinase for antiviral innate immunity response.

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Flow cytometric analysis of Phospho-NAK/TBK1 (S172) expression in HepG2 cells using Phospho-NAK/TBK1 (S172) antibody . Green, isotype control; red, Phospho-NAK/TBK1 (S172).

Immunocytochemical staining of HepG2 cells with Phospho-NAK/TBK1 (S172) antibody . Nuclei were stained blue with DAPI; Phospho-NAK/TBK1 (S172) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using phospho-NAK/TBK1 (S172) antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phospho-NAK/TBK1 (S172) antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Western blotting analysis using phospho-NAK/TBK1 (S172) antibody . Phospho-NAK/TBK1 (S172) expression in wild-type (WT) and TBK1 knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with phospho-NAK/TBK1 (S172) antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

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