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The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

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Western blotting analysis using Phospho-AMPK alpha 1 (S496) antibody . Phospho-AMPK alpha 1 (S496) expression in wild type (WT) and phospho-AMPK alpha 1 (S496) shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with Phospho-AMPK alpha 1 (S496) antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Flow cytometric analysis of Phospho-AMPK alpha 1 (S496) expression in HeLa cells using Phospho-AMPK alpha 1 (S496) antibody . Green, isotype control; red, Phospho-AMPK alpha 1 (S496).

Immunocytochemical staining of HeLa cells with Phospho-AMPK alpha 1 (S496) antibody . Nuclei were stained blue with DAPI; Phospho-AMPK alpha 1 (S496) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using Phospho-AMPK alpha 1 (S496) antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Phospho-AMPK alpha 1 (S496) antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

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