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Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif.

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Western blotting analysis using phospho-Histone H2A.X (S139) antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phospho-Histone H2A.X (S139) antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using phospho-Histone H2A.X (S139) antibody . Phospho-Histone H2A.X (S139) expression in wild-type (WT) and H2AX knockout (KO) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with phospho-Histone H2A.X (S139) antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Flow cytometric analysis of Phospho-Histone H2A.X (S139) expression in HepG2 cells using Phospho-Histone H2A.X (S139) antibody . Green, isotype control; red, Phospho-Histone H2A.X (S139).

Immunocytochemical staining of HepG2 cells with Phospho-Histone H2A.X (S139) antibody . Nuclei were stained blue with DAPI; Phospho-Histone H2A.X (S139) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

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