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The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha subunit and a beta subunit that function in cell surface adhesion and signaling. The encoded preproprotein is proteolytically processed to generate light and heavy chains that comprise the alpha V subunit. This subunit associates with beta 1, beta 3, beta 5, beta 6 and beta 8 subunits. The heterodimer consisting of alpha V and beta 3 subunits is also known as the vitronectin receptor. This integrin may regulate angiogenesis and cancer progression. Alternative splicing results in multiple transcript variants. Note that the integrin alpha 5 and integrin alpha V subunits are encoded by distinct genes.

推荐稀释比 WB: 1/1000-1/5000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000

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Flow cytometric analysis of Integrin subunit alpha V expression in HeLa cells using Integrin subunit alpha V antibody . Green, isotype control; red, Integrin subunit alpha V.

Immunocytochemical staining of HeLa cells with Integrin subunit alpha V antibody . Nuclei were stained blue with DAPI; Integrin subunit alpha V was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using Integrin subunit alpha V antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Integrin subunit alpha V antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using Integrin subunit alpha V antibody . Integrin subunit alpha V expression in wild type (WT) and integrin subunit alpha V shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with Integrin subunit alpha V antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Validation of Integrin subunit alpha V knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Integrin subunit alpha V antibody and analyzed using CytoFLEX.

Immunocytochemical staining of HeLa cells using Integrin subunit alpha V antibody , Top panel: wild-type (WT); Bottom panal: Integrin subunit alpha V shRNA knockdown (KD). Nuclei were stained blue with DAPI; Integrin subunit alpha V was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.

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