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The cysteine protease Atg4 is pivotal to autophagosome membrane generation and regulation. Atg4 primes the Atg8 homologue for lipidation by cleaving its carboxy terminus and exposing its glycine residue for E1-like enzyme Atg7. The Atg8 homologue is transferred to the E2-like enzyme Atg3 before forming the Atg8-PE conjugate. During later stages of autophagy, Atg4 can reverse this lipidation event by cleaving PE, thereby recycling the Atg8 homologue.

推荐稀释比 WB:1/500-1/1000;IHC:1/50-1/100;IP:1/50

Western blot analysis of Atg4A in (1) HepG2 lysates; (2) mouse brain lysates using ATG4A antibody.

-25 ~ -15℃保存，收到货之后有效期1年，避免反复冻融。