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HDAC3 is a nuclear and cytoplasmic protein that deacetylates both histone (H2A, H3, H4) and non-histone substrates (RelA, SRY, p53, MEF2, PCAF and p300/CBP). HDAC3 deacetylase activity is stimulated by interactions with the N-CoR and SMRT co-repressor proteins. Together, these three proteins form a functional complex that represses transcription associated with nuclear hormone receptors and other transcription factors, including Rev-Erb, COUP-TF, DAX1, MAD and Pit-1.

推荐稀释比 WB: 1/1000-1/5000  IHC-P: 1/20-1/50 ICC/IF: 1/200-1/500; FC: 1/20; IP: 1/20-1/50

Western

Western blot analysis of HDAC3 in 3T3, Hela, rat Brain lysates using HDAC3 antibody.

Immunofluorescence micrograph of human lung cancer from paraffin-embedded sections using HDAC3 Rabbit mAb ,dilution 1/100 and Goat Anti-Rabbit IgG H&L (594) ( dilution 1/300) (Red). Nuclear were stained with DAPI (Blue). Heat-induced epitope retrieval was performed using Tris-EDTA buffer (pH 9.0) prior to staining.

-25 ~ -15℃保存，收到货之后有效期1年，避免反复冻融。