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DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of most cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females.

推荐稀释比 WB: 1/500-1/1000  IHC: 1/100-1/200  IF: 1/50-1/200  FC: 1/50-1/100   ELISA: 1/10000

Western

Western blot analysis of MECP2 in  A431 (1) and MCF-7 (2) cell lysate using MECP2 antibody.

Immunohistochemistry

Immunohistochemistry analysis of paraffin-embedded human ovarian cancer tissues using MECP2 antibody.

Immunocytochemistry

Immunocytochemistry analysis of MECP2 in  Hela cells using MECP2 antibody (green),and DAPI(blue). Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Flow

Flow Cytometry analysis of  Hela cells using MECP2 antibody (green) and negative control (red).

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