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Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DGCR8 function as a molecular anchor necessary for the recognition of pri-miRNA at dsRNA-ssRNA junction and directs DROSHA to cleave 11 bp away form the junction to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs.

推荐稀释比 WB: 1/500-1/1000  IF: 1/50-1/200  IP: 1/20

Immunocytochemistry

Immunocytochemistry analysis of DGCR8 (green) in K562 using DGCR8 antibody,and DAPI(blue).

Western

Western blot analysis of DGCR8 in K562, C6, 3T3 lysates using DGCR8 antibody.

-25 ~ -15℃保存，收到货之后有效期1年，避免反复冻融。