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Smac/Diablo is a 21 kDa mammalian mitochondrial protein that functions as a regulatory component during apoptosis. Upon mitochondrial stress, Smac/Diablo is released from mitochondria and competes with caspases for binding of IAPs (inhibitor of apoptosis proteins). The interaction of Smac/Diablo with IAPs relieves the inhibitory effect of the IAPs on caspases.

Western

Western blot analysis of Smac/Diablo in mouse heart, rat skin lysates using SMAC antibody.

Western

Western blot analysis of Smac/Diablo in K562, Hela lysates using Smac/Diablo antibody.

Immunocytochemistry

Immunocytochemistry analysis of Smac/Diablo(green) in Hela using Smac/Diablo antibody,and DAPI(blue)

Immunohistochemistry

Immunohistochemistry analysis of paraffin-embedded Human tonsil using Smac/Diablo antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

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