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Hexokinases I, II, and III are associated with the outer mitochondrial membrane and are critical for maintaining an elevated rate of aerobic glycolysis in cancer cells (Warburg Effect) in order to compensate for the increased energy demands associated with rapid cell growth and proliferation.

Western

Western blot analysis of HK1/HK2 in A431, K562, 231, hela and hepG2 lysates using HK1/HK2 antibody.

Immunohistochemistry

Immunohistochemistry analysis of paraffin-embedded Human lung cancer using HK1/HK2 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

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