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Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis. This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase. Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 and by phosphorylation within the carboxy terminus at Ser473.

Western

Western blot analysis of AKT in 3T3, mouse lung lysates using AKT antibody.

Western

Western blot analysis of AKT1/2/3 in Jurkat, rat Brain, C6, CHO-K1, Hela lysates using AKT antibody.

Immunohistochemistry

Immunohistochemistry analysis of paraffin-embedded Human lung cancer using AKT1/2/3 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

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