推荐应用
标签抗原由于其分子量小,对融合蛋白的活性没有影响等特点,已被广泛应用于重组蛋白在后续免疫印迹、免疫沉淀和免疫技术的检测中。Flag标签是重组蛋白研究中应用较为广泛的一种,该标签系统利用一个短的亲水性八氨基酸肽(DYKDDDDK)融合到目标蛋白的N-端或者C-端,且该标签可被后续肠激酶去除。Flag抗体可以用于检测和Flag标签融合表达蛋白的表达、细胞内定位,以及纯化、定性或定量检测Flag融合表达蛋白等。
建议稀释浓度:WB: 1/500-1/5000; IF: 1/200; IP: 1/5000; ELISA: 1/500-1/5000
Western blot

Western blot analysis of different concentration of Flag-Tag (DYKDDDDK) , Mouse mAb.
Antigen: Extracts from E.coli expressing Flag-tagged protein Line No.1: 1 μg/mL;2: 0.5 μg/mL;3: 0.1 μg/mL
Immunoprecipitation (IP)

Immunoprecipitation (IP) of Flag-tagged fusion proteins was performed using Flag-Tag Mouse mAb (30503ES) conjugated to agarose beads, followed by Western blot detection with the same antibody at dilutions ranging from 1:5000 to 1:20000.
Immunofluorescence

Immunofluorescence analysis of the Flag tag in Flag-tagged protein-transfected 293T cells was performed using Flag-Tag antibody (green) and DAPI (blue) at a dilution ratio of 1:200.
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