永利3044(中国集团)有限公司官网

Abstract

Circular RNAs (circRNAs) have been demonstrated to be involved in osteosarcoma (OS) development; however, the underlying mechanism of circKMT2D in OS progression remains unclear. The present study aimed to elucidate how circKMT2D could affect hydrogen peroxide (H₂O₂)‑induced OS progression. H₂O₂ (100 µmol/l) was used to treat MG63 and U2OS cells. The cell viability, invasive ability, apoptosis and circKMT2D expression were detected using Cell Counting Kit‑8 assay, Transwell assay, flow cytometry and reverse transcription‑quantitative PCR, respectively. Furthermore, MG63 and U2OS cells transfected with circKMT2D short hairpin RNA and negative control were treated with H₂O₂, and circKMT2D expression and cell phenotype were determined. Dual‑luciferase reporter assay was conducted to determine the association between circKMT2D and miR‑210 expression level. Rescue experiments were conducted to examine the mechanisms through which circKMT2D and miR‑210 could affect H₂O₂‑treated MG63 cells. In addition, the effects of miR‑210 on the expression of the autophagy‑related proteins Beclin1 and p62 in H₂O₂‑treated MG63 cells were detected by western blotting. An autophagy inhibitor was used to treat the MG63 cells, and whether miR‑210 could affect the H₂O₂‑treated MG63 cell phenotype through autophagy was investigated. The results demonstrated that H₂O₂ treatment promoted cell apoptosis and decreased cell viability, invasive ability and circKMT2D expression in MG63 and U2OS cells. Furthermore, circKMT2D knockdown decreased the cell viability and invasive ability and enhanced the apoptosis of H₂O₂‑treated MG63 and U2OS cells. circKMT2D possessed binding sites for miR‑210 and inhibited miR‑210 expre